In addition to cell division and furrow formation, inhibition of Myo1 has a drastic effect on the dynamics and distribution of lipid droplets (LDs) in the blastodisc near the cleavage furrow. Myo1 appears to be crucial for maintaining the blastodisc morphology and the actin cortex thickness. We found that the blastodisc of the embryo, which contains a thick actin cortex (~13 μm), is loaded with cor-tical Myo1. While Myo1 isoforms appears to be important for both the formation and the maintenance of cleavage furrows, Myo2 is required only for the formation of furrows. Inhibition of Myo1 (by PClP) and Myo2 (by Blebbistatin) lead to arrest in cell division. Our results indicate a unique involvement of Myo1 in early development of Zebra-fish embryos. We have studied the effect of Myo1 inhibitor PClP in 1–8 cell Zebrafish embryos. Myosin-1 (Myo1) represents a mechanical link between the membrane and actin-cytoskele-ton in animal cells.
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